DNA
Scar25 ATG

Part:BBa_K1319106:Design

Designed by: Michael Osthege   Group: iGEM14_Aachen   (2014-10-03)


RFC 25 Translation Start Scar Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc).


Source

This sequence is produced by an assembly method.

References